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Croda International Plc d 7 7 keto cholesterol
D 7 7 Keto Cholesterol, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 181 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/d+7+cholesterol/cholesterol-d7/pmc12955076-300-19-42
Average 95 stars, based on 181 article reviews
d 7 7 keto cholesterol - by Bioz Stars, 2026-11
95/100 stars

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Article Title: Specialization and adaptation in pollen sterol use by wild bees
Article Snippet: d 7 -cholesterol , Avanti polar lipids (Alabama, USA). , CAS No:83199-47-7.

Article Title: Cholesterol promotes both head group visibility and clustering of PI(4,5)P 2 driving unconventional secretion of Fibroblast Growth Factor 2
Article Snippet: Lipid standards were added prior to extractions, using a master mix containing 50 pmol phosphatidylcholine (PC)-standard mix (PC 13:0/13:0, 14:0/14:0, 2.0:0/20:0; 21:0/21:0; Avanti Polar Lipids) and 150 pmol D 7 -cholesterol (Avanti Polar Lipids).

Clinical Proteomics:

Article Title: Obesity-Associated Hyperuricemia in Female Mice: A Reevaluation
Article Snippet: .. To analyze cholesterol, cholesteryl esters, and triglycerides in plasma samples, 15 μL of each plasma sample was spiked with 5 pmol of d 7 -cholesterol (Avanti Polar Lipids, Inc.), 5 pmol of d 7 -16:0-cholesteryl ester (Avanti Polar Lipids, Inc.), and 19.7 pmol of TG17:0/17:0/17:0 (Avanti Polar Lipids, Inc.) as internal standards for cholesterol, cholesteryl esters, and triglycerides, respectively. ..

Targeted Proteomics:

Article Title: Stable reduction of STARD4 alters cholesterol regulation and lipid homeostasis
Article Snippet: An Agilent Zorbax Rx-Sil column (inner diameter 2.1 x 100 mm) was used under the following conditions: mobile phase A (chloroform:methanol:1 M ammonium hydroxide, 89.9:10:0.1, v/v) and mobile phase B (chloroform:methanol:water:ammonium hydroxide, 55:39.9:5:0.1, v/v); 95% A for 2 min, linear gradient to 30% A over 18 min and held for 3 min, and linear gradient to 95% A over 2 min and held for 6 min. Sterols and glycerolipids were separated with reverse-phase HPLC using an isocratic mobile phase as before [ 34 ] except with an Agilent Zorbax Eclipse XDB-C18 column (4.6 x 100 mm). .. Quantification of lipid species was accomplished using multiple reaction monitoring (MRM) transitions that were developed in earlier studies [ 34 - 36 ] in conjunction with referencing of appropriate internal standards: PA 14:0/14:0, PC 14:0/14:0, PE 14:0/14:0, PG 15:0/15:0, PI 16:0/16:0, PS 14:0/14:0, BMP 14:0/14:0, APG 14:0/14:0, LPC 17:0, LPE 14:0, LPI 13:0, Cer d18:1/17:0, SM d18:1/12:0, dhSM d18:0/12:0, GalCer d18:1/12:0, GluCer d18:1/12:0, LacCer d18:1/12:0, D 7 -cholesterol, CE 17:0, MG 17:0, 4ME 16:0 diether DG, D 5 -TG 16:0/18:0/16:0 (Avanti Polar Lipids, Alabaster, AL). ..

Article Title: Decreased surfactant lipids correlate with lung function in chronic obstructive pulmonary disease (COPD)
Article Snippet: An Agilent Zorbax Rx-Sil column (inner diameter 2.1 x 100 mm) was used under the following conditions: mobile phase A (chloroform:methanol:1 M ammonium hydroxide, 89.9:10:0.1, v/v) and mobile phase B (chloroform:methanol:water:ammonium hydroxide, 55:39.9:5:0.1, v/v); 95% A for 2 min, linear gradient to 30% A over 18 min and held for 3 min, and linear gradient to 95% A over 2 min and held for 6 min. Sterols and glycerolipids were separated with reverse-phase HPLC using an isocratic mobile phase as before [ ] except with an Agilent Zorbax Eclipse XDB-C18 column (4.6 x 100 mm). .. Quantification of lipid species was accomplished using multiple reaction monitoring (MRM) transitions and instrument settings that were developed in earlier studies [ ] in conjunction with referencing of appropriate internal standards PA 14:0/14:0, PC 14:0/14:0, PE 14:0/14:0, PG 15:0/15:0, PI 17:0/20:4, PS 14:0/14:0, BMP 14:0/14:0, APG 14:0/14:0, LPC 17:0, LPE 14:0, LPI 13:0, Cer d18:1/17:0, SM d18:1/12:0, dhSM d18:0/12:0, GalCer d18:1/12:0, GluCer d18:1/12:0, LacCer d18:1/12:0, D 7 -cholesterol, CE 17:0, MG 17:0, 4ME 16:0 diether DG, D 5 -TG 16:0/18:0/16:0 (Avanti Polar Lipids, Alabaster, AL). ..

Article Title: Mitofusins regulate lipid metabolism to mediate the development of lung fibrosis
Article Snippet: An Agilent Zorbax Rx-Sil column (inner diameter 2.1 × 100 mm) was used under the following conditions: mobile phase A (chloroform:methanol:1 M ammonium hydroxide, 89.9:10:0.1, v/v) and mobile phase B (chloroform:methanol:water:ammonium hydroxide, 55:39.9:5:0.1, v/v); 95% A for 2 min, linear gradient to 30% A over 18 min and held for 3 min, and linear gradient to 95% A over 2 min and held for 6 min. Sterols and glycerolipids were separated with reverse-phase HPLC using an isocratic mobile phase as before except with an Agilent Zorbax Eclipse XDB-C18 column (4.6 × 100 mm). .. Quantification of lipid species was accomplished using multiple reaction monitoring (MRM) transitions that were developed in earlier studies in conjunction with referencing of appropriate internal standards: PA 14:0/14:0, PC 14:0/14:0, PE 14:0/14:0, PI 12:0/13:0, PS 14:0/14:0, SM d18:1/12:0, D 7 -cholesterol, CE 17:0, MG 17:0, 4ME 16:0 diether DG, D 5 -TG 16:0/18:0/16:0 (Avanti Polar Lipids, Alabaster, AL). ..

Tissue:

Article Title: Obese female mice do not exhibit overt hyperuricemia despite hepatic steatosis and impaired glucose tolerance
Article Snippet: .. Liver tissue homogenates were spiked with d 7 -Cholesterol and 16:0-d 7 -cholesteryl ester (Avanti Polar Lipids, Al) as internal standards. ..



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Cambridge Isotope Laboratories 25,26,26,26,27,27,27- 2 h 7 ]cholesterol (d 7 -cholesterol)
Experimental paradigms for different measurements. A: Tissue sterol levels in hamsters on a normal diet (ND) plus fat- and <t>cholesterol-containing</t> oral gavage (FCCOG). B: Tissue sterol levels in mice on ND. C: Tissue cholesterol input comprised of in situ biosynthesis and uptake of the synthesized cholesterol from the systemic circulation. D: Relative deuterium enrichment of 24-hydroxycholesterol. E: Tissue uptake of dietary cholesterol. D 2 O, deuterated water; D 7 -FCCOG, FCCOG containing deuterated cholesterol.
25,26,26,26,27,27,27 2 H 7 ]Cholesterol (D 7 Cholesterol), supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Experimental paradigms for different measurements. A: Tissue sterol levels in hamsters on a normal diet (ND) plus fat- and cholesterol-containing oral gavage (FCCOG). B: Tissue sterol levels in mice on ND. C: Tissue cholesterol input comprised of in situ biosynthesis and uptake of the synthesized cholesterol from the systemic circulation. D: Relative deuterium enrichment of 24-hydroxycholesterol. E: Tissue uptake of dietary cholesterol. D 2 O, deuterated water; D 7 -FCCOG, FCCOG containing deuterated cholesterol.

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: Experimental paradigms for different measurements. A: Tissue sterol levels in hamsters on a normal diet (ND) plus fat- and cholesterol-containing oral gavage (FCCOG). B: Tissue sterol levels in mice on ND. C: Tissue cholesterol input comprised of in situ biosynthesis and uptake of the synthesized cholesterol from the systemic circulation. D: Relative deuterium enrichment of 24-hydroxycholesterol. E: Tissue uptake of dietary cholesterol. D 2 O, deuterated water; D 7 -FCCOG, FCCOG containing deuterated cholesterol.

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: In Situ, Synthesized

Effects of the fat- and cholesterol-containing oral gavage (FCCOG). A: Serum lipid profile measured by a clinical laboratory. B–D: Tissue sterols quantified by GS-MS. All data represent the mean ± SD of the measurements in individual hamsters; the number of animals (n) for each panel is indicated in the figure. Statistical significance was assessed by a two-tailed, unpaired Student’s t test. ∗ P ≤ 0.05; ∗∗ P ≤ 0.01; ∗∗∗ P ≤ 0.001. 27COOH, cholestenoic acid; 27HC, 27-hydroxycholesterol; HDL, high density lipoprotein cholesterol; LDL, low density lipoprotein cholesterol; TG, triglycerides.

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: Effects of the fat- and cholesterol-containing oral gavage (FCCOG). A: Serum lipid profile measured by a clinical laboratory. B–D: Tissue sterols quantified by GS-MS. All data represent the mean ± SD of the measurements in individual hamsters; the number of animals (n) for each panel is indicated in the figure. Statistical significance was assessed by a two-tailed, unpaired Student’s t test. ∗ P ≤ 0.05; ∗∗ P ≤ 0.01; ∗∗∗ P ≤ 0.001. 27COOH, cholestenoic acid; 27HC, 27-hydroxycholesterol; HDL, high density lipoprotein cholesterol; LDL, low density lipoprotein cholesterol; TG, triglycerides.

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: Two Tailed Test

A summary of experimental data and calculations

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: A summary of experimental data and calculations

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: Concentration Assay, In Situ

Tissue relative enrichments after 2 weeks of either D 2 O or D 7 -FCCOG administration. A: Whole-body water deuterium ([ 2 H]) enrichment from D 2 O. B: Tissue [ 2 H] enrichment from D 2 O. C: Tissue D 7 -cholesterol enrichment from D 7 fat- and cholesterol-containing oral gavage FCCOG. All data represent the means ± S.D. of the measurements in individual hamsters; the number of animals (n) is indicated in each panel.

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: Tissue relative enrichments after 2 weeks of either D 2 O or D 7 -FCCOG administration. A: Whole-body water deuterium ([ 2 H]) enrichment from D 2 O. B: Tissue [ 2 H] enrichment from D 2 O. C: Tissue D 7 -cholesterol enrichment from D 7 fat- and cholesterol-containing oral gavage FCCOG. All data represent the means ± S.D. of the measurements in individual hamsters; the number of animals (n) is indicated in each panel.

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques:

A comparison of  cholesterol  input to the retina and brain in hamsters and mice

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: A comparison of cholesterol input to the retina and brain in hamsters and mice

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: Comparison, Concentration Assay, In Situ

Correlation analyses. A: Relative deuterium ([ 2 H]) enrichment of brain 24-hydroxycholesterol (24HC) versus relative [ 2 H] enrichment of brain cholesterol. B: Relative [ 2 H] enrichment of brain 24HC versus relative [ 2 H] enrichment of plasma 24HC. C: Relative [ 2 H] enrichment of brain cholesterol versus relative [ 2 H] enrichment of plasma 24HC. Each dot represents the measurements in individual hamster, a total of 10 measurements (hamsters) in each panel: 4 animals were on D 2 O for 13 days, 3 animals were on D 2 O for 24 days, and 3 animals were on D 2 O for 35 days. A Pearson correlation coefficient and P value were computed to assess the linear relationship between the two variables in each panel. Data were fit by simple linear regression; the regression equations are indicated in each panel.

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: Correlation analyses. A: Relative deuterium ([ 2 H]) enrichment of brain 24-hydroxycholesterol (24HC) versus relative [ 2 H] enrichment of brain cholesterol. B: Relative [ 2 H] enrichment of brain 24HC versus relative [ 2 H] enrichment of plasma 24HC. C: Relative [ 2 H] enrichment of brain cholesterol versus relative [ 2 H] enrichment of plasma 24HC. Each dot represents the measurements in individual hamster, a total of 10 measurements (hamsters) in each panel: 4 animals were on D 2 O for 13 days, 3 animals were on D 2 O for 24 days, and 3 animals were on D 2 O for 35 days. A Pearson correlation coefficient and P value were computed to assess the linear relationship between the two variables in each panel. Data were fit by simple linear regression; the regression equations are indicated in each panel.

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: Clinical Proteomics

A comparison of the whole-body  cholesterol  maintenance in mice, hamsters, and humans

Journal: Journal of Lipid Research

Article Title: Quantitative characterizations of the cholesterol-related pathways in the retina and brain of hamsters

doi: 10.1016/j.jlr.2023.100401

Figure Lengend Snippet: A comparison of the whole-body cholesterol maintenance in mice, hamsters, and humans

Article Snippet: [25,26,26,26,27,27,27- 2 H 7 ]Cholesterol (D 7 -cholesterol) was purchased from Cambridge Isotope Laboratories Inc., [25,26,26,26,27,27,27- 2 H 7 ]-sitosterol (D 7 -sitosterol) was from Toronto Research Chemicals Inc. [1,2,5,6- 2 H 4 ]Lathosterol (D 4 -lathosterol), [26,26,26,27,27,27- 2 H 6 ]desmosterol (D 6 -desmosterol), [25,26,26,26,27,27,27- 2 H 7 ]24HC and [26,26,26,27,27- 2 H 5 ]27HC were from CDN Isotopes (Pointe-Claire, Canada), 23,24-bisnor-5-cholenic acid-3β-ol from Steraloids (Newport, RI).

Techniques: Comparison, Expressing, In Situ